Biochemistry
Ehsan Shariat; Ali Dalir Ghaffari; Somayyeh Mosavipoor; Somayyeh Namroodi; Javid Sadraie
Abstract
Background and aims: Toxoplasmosis is a very common disease in the world and has two types,including chronic and acute toxoplasmosis. In the chronic toxoplasmosis, the abnormality of tissuefunction is negligible, but in acute toxoplasmosis, the function of the reticuloendothelial system isinterrupted ...
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Background and aims: Toxoplasmosis is a very common disease in the world and has two types,including chronic and acute toxoplasmosis. In the chronic toxoplasmosis, the abnormality of tissuefunction is negligible, but in acute toxoplasmosis, the function of the reticuloendothelial system isinterrupted and the assessment of Toxoplasma antibody with tissue enzymes is very essential in thisstage. In addition, in some conditions, serum ferritin increases in the acute phase of the infectiousdisease. In congenital toxoplasmosis, the evaluation of biochemical factors and IgG avidity test isimportant for detecting the acute congenital toxoplasmosis in pregnant women. Based on theabove-mentioned explanations, the present study aimed to evaluate the biochemical factors inpatients with acute toxoplasmosis (congenital toxoplasmosis) among the Iranian population usingelectrochemiluminescence and IgG ELISA avidity.Methods: The study was based on a comparative abundance study and was conducted from March toJune 2017. Material included 980 serum and amniotic fluid samples collected from human blood witha high level of IgG antibody against Toxoplasma gondii in Rajaie Center, Tehran, Iran. The standard andthe main tests included the ELISA assay and the measurement of the liver transaminases (i.e., SGOTand SGPT), along with/namely bilirubin and ferritin used to detect IgG antibodies and to evaluate theacute toxoplasmosis, respectively. Finally, the results were analyzed by SPSS software.Results: The results showed that the level of liver transaminases, namely, serum bilirubin and ferritinincrease in some patients with a high level of IgG avidity antibody against Toxoplasma gondii. Forexample, the mean serum levels of SGOT was 108 IU/L in 120 patients with acute toxoplasmosis andthe mean serum bilirubin was about 5 mg/dL in 80 patients.Conclusion: Overall, in acute congenital toxoplasmosis, the evaluation of IgG AVIDITY is regarded asthe first step and then the measurement of biochemical factors such as serum transaminases, serumbilirubin, and serum ferritin is important.
Infectious Disease
Javid Sadraie; Ehsan Shariat Bahadory; Sekhavat Ghaffari Novin
Volume 3, Issue 1 , February 2016, , Pages 3-11
Abstract
Background and aims: Toxoplasmosis is a parasitic disease which may cause some laboratory symptoms in infected individuals. One of the main ways of transmition this organism is placenta to fetus pathway. If this transmission occurs in the 3th month of pregnancy, the abortion, central nerve system and ...
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Background and aims: Toxoplasmosis is a parasitic disease which may cause some laboratory symptoms in infected individuals. One of the main ways of transmition this organism is placenta to fetus pathway. If this transmission occurs in the 3th month of pregnancy, the abortion, central nerve system and ocular disorder will happen. Because of this issue, the precise technique for detection of Toxoplasma Antibody such as IgG and IgM is important, that contains ELISA to detect Toxoplasma Antibody such as IgG and IgM and AFP.Methods: This was a cross sectional study. In this study, the main sample was serum that was randomly collected from 255 pregnant women infected with toxoplasma Gondi in Avesina center. Then, It was detected the serum concentration of AFP in toxoplasmosis pregnant women with high level of IgG and IgM toxoplasma antibody by ELISA assay.Results: The results of this survey showed that the infection in these pregnant women by toxoplasma gondii was occurred and 13% of them (13% of 255 infected mothers) had high levels of AFP in their serum. The statistical analyses was done by SPSS consisted of t-test, case number, comparative study, and Q-Q plot evaluations.Conclusions: In some pregnant women with high level of IgG and IgM toxoplasma antibody was observed in high levels of AFP in their serum and this index correlates with NTD in their fetus.
Infectious Disease
Somayyeh Namroodi; Ehsan Shariat Bahadory
Volume 2, Issue 4 , November 2015, , Pages 190-196
Abstract
Background and aims: Cats carry a lot of zoonotic parasites such as Toxoplasma gondii (T. gondii) which causes toxoplasmosis in warm-blooded animals. Toxoplasmosis includes wide range of symptoms in its intermediate and definitive hosts but the worst symptoms have been seen in pregnant women and also ...
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Background and aims: Cats carry a lot of zoonotic parasites such as Toxoplasma gondii (T. gondii) which causes toxoplasmosis in warm-blooded animals. Toxoplasmosis includes wide range of symptoms in its intermediate and definitive hosts but the worst symptoms have been seen in pregnant women and also on immunocompromised hosts. Considering the importance of the fact that cats are as the only definitive host that spread T. gondii oocytes in environment, it is necessary to obtain comprehensive information about the role of cats in life cycle of T. gondii.Methods: This comparative study was done during the January 2012 to February 2014, a total of 210 feral cats were captured by hand made traps. Age, sex, season and locality were documented. Blood samples from carotid vein were collected. Sera were separated by centrifugation at 3000 rpm for 10 min and stored at 20oC until laboratory examination. Serum sample of 210 feral cats from 20 villages of Golestan province were obtained and analyzed for presence of Ig G antibody against T. gondii by ELIZA Kit.Results: The frequency of T. gondii infection in serum samples was 85% (180/210). Percentage of T. gondii infection was similar in male and female and different seasons but significant difference was recorded between sampled villages. Also the seropositivity rate of T. gondii had positive relationship with age (P˂0.005).Conclusion: High seropositive results of the current study highlight the risk of endemic toxoplasmosis in the sampled rural ecosystem. Performing suitable screening test in rural population, keeping feral cats far from the villages, controlling of feral cats population by sterilization and making villagers aware of toxoplasmosis danger are recommended.