Virology
Mazaher Khodabandehloo; Pezhman Sharifi
Abstract
Background and aims: Maternal primary and recurrent infection of cytomegalovirus (CMV) may be transmitted to the fetus during pregnancy and may have complications such as death or growth, along with the development retardation of the fetus and infant. The aim of this study was to determine ...
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Background and aims: Maternal primary and recurrent infection of cytomegalovirus (CMV) may be transmitted to the fetus during pregnancy and may have complications such as death or growth, along with the development retardation of the fetus and infant. The aim of this study was to determine the prevalence of immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies against CMV in young women, Sanandaj, Iran.Methods: To this end, 90 women (15-40 years old) referring to a clinical laboratory were randomly selected and announced their informed consent to participate in this cross-sectional study. Demographic information and women’s data were collected, including pregnancy, history of abortion, and history of blood transfusion. Then, women’s sera were measured for CMV IgG and IgM antibodies using the electrochemiluminescence technique. Finally, the data were analyzed by SPSS statistical software.Results: The prevalence of IgG and IgM antibodies against CMV in women was 92.2% (95% CI = 86.5-97.8) and 0%, respectively. In addition, the average CMV IgG antibody level was about 137.52 ± 85.215 SD IU/mL. The results revealed a significant statistical association between IgG antibody and pregnancy (P value = 0.012) while there was no association between CMV IgG antibody and other demographic data.Conclusions: In general, high percentages of women had CMV IgG antibody whereas 7.8% of them were susceptible. They are expected to acquire CMV primary infection, and therefore, the screening of antibodies to CMV is suggested for prenatal care.
Biochemistry
Ehsan Shariat; Ali Dalir Ghaffari; Somayyeh Mosavipoor; Somayyeh Namroodi; Javid Sadraie
Abstract
Background and aims: Toxoplasmosis is a very common disease in the world and has two types,including chronic and acute toxoplasmosis. In the chronic toxoplasmosis, the abnormality of tissuefunction is negligible, but in acute toxoplasmosis, the function of the reticuloendothelial system isinterrupted ...
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Background and aims: Toxoplasmosis is a very common disease in the world and has two types,including chronic and acute toxoplasmosis. In the chronic toxoplasmosis, the abnormality of tissuefunction is negligible, but in acute toxoplasmosis, the function of the reticuloendothelial system isinterrupted and the assessment of Toxoplasma antibody with tissue enzymes is very essential in thisstage. In addition, in some conditions, serum ferritin increases in the acute phase of the infectiousdisease. In congenital toxoplasmosis, the evaluation of biochemical factors and IgG avidity test isimportant for detecting the acute congenital toxoplasmosis in pregnant women. Based on theabove-mentioned explanations, the present study aimed to evaluate the biochemical factors inpatients with acute toxoplasmosis (congenital toxoplasmosis) among the Iranian population usingelectrochemiluminescence and IgG ELISA avidity.Methods: The study was based on a comparative abundance study and was conducted from March toJune 2017. Material included 980 serum and amniotic fluid samples collected from human blood witha high level of IgG antibody against Toxoplasma gondii in Rajaie Center, Tehran, Iran. The standard andthe main tests included the ELISA assay and the measurement of the liver transaminases (i.e., SGOTand SGPT), along with/namely bilirubin and ferritin used to detect IgG antibodies and to evaluate theacute toxoplasmosis, respectively. Finally, the results were analyzed by SPSS software.Results: The results showed that the level of liver transaminases, namely, serum bilirubin and ferritinincrease in some patients with a high level of IgG avidity antibody against Toxoplasma gondii. Forexample, the mean serum levels of SGOT was 108 IU/L in 120 patients with acute toxoplasmosis andthe mean serum bilirubin was about 5 mg/dL in 80 patients.Conclusion: Overall, in acute congenital toxoplasmosis, the evaluation of IgG AVIDITY is regarded asthe first step and then the measurement of biochemical factors such as serum transaminases, serumbilirubin, and serum ferritin is important.