Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)

Authors

1 Department of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modaress University, Tehran, Iran

2 Assistant professor, Department of Environmental sciences, Faculty of Fisheries and Environmental Sciences, University of Agricultural Sciences & Natural Resources, Gorgan, Iran

3 Department of Nursing, Faculty of Medical Sciences, Tehran Medical Sciences University, Tehran, Iran

4 Department of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Background and aims: Toxoplasmosis is a very common disease in the world. Two types of acquired
toxoplasmosis have been detected. In the chronic toxoplasmosis, the abnormality of tissue function
is little but, in acute toxoplasmosis, function of RES system becomes interrupted. The assessment of
toxoplasma antibody with tissue enzymes in this stage is very important. Furthermore, serum ferritin
in some conditions became high in acute phase of infectious disease.
Methods: This study was based on comparative abundance study. Materials consisted of 980 serum
and amniotic fluid samples collected from human blood with high level of IgG antibody against
Toxoplasma gondii in Rajaie center, Tehran, Iran. The standard test was ELISA assay to detect these
antibodies and the main test was measurement of liver transaminases (SGOT, SGPT) bilirubin and
ferritin to evaluate acute toxoplasmosis. Data analysis was done by SPSS version 18.0. This study was
done during March to June, 2017.
Results: Results showed that in some patients with high level of IgG AVIDITY antibody against T. gondii
the level of liver transaminases, serum bilirubin and ferritin became increased. For example in 120
patients with acute toxoplasmosis the mean serum levels of SGOT were 108 IU/L and in 80 patients
the mean serum bilirubin were about 5 mg/dL.
Conclusion: In acute congenital toxoplasmosis, the evaluation of IgG AVIDITY was first step and then
the measurement of biochemical factors such as serum transaminases, serum Bilirubin and serum
Ferritin were important.